![]() In MAS practice, it is a common problem that cultivars exhibiting desirable marker genotypes may not necessarily have the targeted genes or alleles and vice versa, which is known as “false positives.” The aim of this study was to compare the available four markers: Xwmc44, Xgwm259, Xbarc80, and csLV46G22 markers (not published yet), for the identification of the Lr46/Yr29 loci in 73 genotypes of wheat, which were reported as sources of various “slow rusting” genes, including 60 with confirmed Lr46/Yr29 gene, reported in the literature. Until now, eight genes ( Lr34/Yr18, Lr46/Yr29, Lr67/Yr46, Lr68, Lr74, Lr75, Lr77, and Lr78 ) conferring resistance against multiple fungal pathogens have been identified in wheat gene pool and the molecular markers were developed for them. ![]() Currently, one of the major challenges of wheat geneticists and breeders is to accumulate major genes for durability of rust resistance called “slow rusting” genes using marker-assisted selection (MAS). Wheat leaf rust, caused by fungal pathogen Puccinia triticina Erikss, annually contributes to production losses as high as 40% in susceptible varieties and remains as one of the most damaging diseases of wheat worldwide.
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